› 3twd

X-ray diffraction
1.9Å resolution

glmuC1 in complex with an antibacterial inhibitor

Released:
DOI: 10.2210/pdb3twd/pdb
PDB entry 3twd coloured by chain, front view.
PDB entry 3twd coloured by chain, side view.
PDB entry 3twd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
In vitro validation of acetyltransferase activity of GlmU as an antibacterial target in Haemophilus influenzae.
Buurman ET, Andrews B, Gao N, Hu J, Keating TA, Lahiri S, Otterbein LR, Patten AD, Stokes SS, Shapiro AB
J Biol Chem 286 40734-42 (2011)
PMID: 21984832

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-142259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein GlmU Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 23.5 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACC7 (Residues: 233-452; Coverage: 48%)
Gene names: JW3708, b3730, glmU, yieA
Sequence domains: Bacterial transferase hexapeptide (six repeats)
Structure domains: Hexapeptide repeat proteins

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOB 2 x GOB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ DW
Spacegroup: P63
Unit cell:
a: 80.526Å b: 80.526Å c: 139.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
not available not available 0.217
Expression system: Escherichia coli

Quick links

Citations

3 review citations

ESKAPEing the labyrinth of antibacterial discovery.
Tommasi et al. (2015)
2 more

3 mentions without citation

In vitro validation of acetyltransferase activity of GlmU as an antibacterial target in Haemophilus influenzae.
Buurman et al. (2011)
2 more