› 3tnl

X-ray diffraction
1.45Å resolution

1.45 Angstrom Crystal Structure of Shikimate 5-dehydrogenase from Listeria monocytogenes in Complex with Shikimate and NAD.

Released:
DOI: 10.2210/pdb3tnl/pdb
PDB entry 3tnl coloured by chain, front view.
PDB entry 3tnl coloured by chain, side view.
PDB entry 3tnl coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Entry authors: Minasov G, Light SH, Halavaty A, Shuvalova L, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 315 amino acids
Theoretical weight: 34.94 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8Y9N5 (Residues: 1-291; Coverage: 100%)
Gene names: aroE, lmo0490
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand SKM 2 x SKM
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 71.851Å b: 82.878Å c: 101.26Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.15 0.149 0.176
Expression system: Escherichia coli BL21(DE3)

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