› 3tkl

X-ray diffraction
2.18Å resolution

Crystal structure of the GTP-bound Rab1a in complex with the coiled-coil domain of LidA from Legionella pneumophila

Released:
DOI: 10.2210/pdb3tkl/pdb
PDB entry 3tkl coloured by chain, front view.
PDB entry 3tkl coloured by chain, side view.
PDB entry 3tkl coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into a unique Legionella pneumophila effector LidA recognizing both GDP and GTP bound Rab1 in their active state.
Cheng W, Yin K, Lu D, Li B, Zhu D, Chen Y, Zhang H, Xu S, Chai J, Gu L
PLoS Pathog 8 e1002528 (2012)
PMID: 22416225

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158707 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-1A Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 21.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62820 (Residues: 1-191; Coverage: 93%)
Gene names: RAB1, RAB1A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
LidA protein, substrate of the Dot/Icm system Chain: B
Molecule details ›
Chain: B
Length: 267 amino acids
Theoretical weight: 30.63 KDa
Source organism: Legionella pneumophila str. Corby
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 128.823Å b: 49.709Å c: 61.702Å
α: 90° β: 99.27° γ: 90°
R-values:
R R work R free
0.194 0.191 0.252
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Formation of a pathogen vacuole according to Legionella pneumophila: how to kill one bird with many stones.
Finsel et al. (2015)
9 more

8 mentions without citation

Rab GTPases: Emerging Oncogenes and Tumor Suppressive Regulators for the Editing of Survival Pathways in Cancer.
Gopal Krishnan et al. (2020)
7 more