› 3ta0

X-ray diffraction
2.3Å resolution

A. fulgidus GlnK3, MgATP complex

Released:
DOI: 10.2210/pdb3ta0/pdb
PDB entry 3ta0 coloured by chain, front view.
PDB entry 3ta0 coloured by chain, side view.
PDB entry 3ta0 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.
Maier S, Schleberger P, Lü W, Wacker T, Pflüger T, Litz C, Andrade SL
PLoS One 6 e26327 (2011)
PMID: 22039461

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128035 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein GlnK3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 118 amino acids
Theoretical weight: 13.16 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O28524 (Residues: 12-120; Coverage: 91%)
Gene names: AF_1750, glnK3
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand ATP 6 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 123.751Å b: 93.626Å c: 88.644Å
α: 90° β: 133.55° γ: 90°
R-values:
R R work R free
0.237 0.235 0.267
Expression system: Escherichia coli K-12

Quick links

Citations

4 review citations

P(II) signal transduction proteins: nitrogen regulation and beyond.
Huergo et al. (2013)
3 more

1 mention without citation

Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.
Maier et al. (2011)