› 3t9u

X-ray diffraction
1.97Å resolution

CNQX bound to an oxidized double cysteine mutant (A452C/S652C) of the ligand binding domain of GluA2

Released:
DOI: 10.2210/pdb3t9u/pdb
PDB entry 3t9u coloured by chain, front view.
PDB entry 3t9u coloured by chain, side view.
PDB entry 3t9u coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Mechanism of AMPA receptor activation by partial agonists: disulfide trapping of closed lobe conformations.
Ahmed AH, Wang S, Chuang HH, Oswald RE
J Biol Chem 286 35257-66 (2011)
PMID: 21846932

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 258 amino acids
Theoretical weight: 28.88 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 414-527, 653-794; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand CNI 3 x CNI
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 114.724Å b: 165.243Å c: 47.651Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.219
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structural and Functional Architecture of AMPA-Type Glutamate Receptors and Their Auxiliary Proteins.
Greger et al. (2017)
7 more

1 mention without citation

Mechanism of AMPA receptor activation by partial agonists: disulfide trapping of closed lobe conformations.
Ahmed et al. (2011)