› 3t4d

X-ray diffraction
1.7Å resolution

Crystal Structure of NaK2K Channel Y55F Mutant

Released:
DOI: 10.2210/pdb3t4d/pdb
PDB entry 3t4d coloured by chain, front view.
PDB entry 3t4d coloured by chain, side view.
PDB entry 3t4d coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Protein interactions central to stabilizing the K+ channel selectivity filter in a four-sited configuration for selective K+ permeation.
Sauer DB, Zeng W, Raghunathan S, Jiang Y
Proc Natl Acad Sci U S A 108 16634-9 (2011)
PMID: 21933962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.76 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 20-110; Coverage: 80%)
Gene name: BC_0669
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand K 8 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I4
Unit cell:
a: 67.439Å b: 67.439Å c: 83.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.225
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.
Payandeh et al. (2015)
6 more

2 mentions without citation

Protein interactions central to stabilizing the K+ channel selectivity filter in a four-sited configuration for selective K+ permeation.
Sauer et al. (2011)
1 more