› 3sv5

X-ray diffraction
1.53Å resolution

Engineered medium-affinity halide-binding protein derived from YFP: iodide complex

Released:
DOI: 10.2210/pdb3sv5/pdb
PDB entry 3sv5 coloured by chain, front view.
PDB entry 3sv5 coloured by chain, side view.
PDB entry 3sv5 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.
Grimley JS, Li L, Wang W, Wen L, Beese LS, Hellinga HW, Augustine GJ
J Neurosci 33 16297-309 (2013)
PMID: 24107961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.31 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand IOD 4 x IOD
Ligand FMT 1 x FMT
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P212121
Unit cell:
a: 51.488Å b: 63.025Å c: 66.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.182
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Cation-chloride cotransporters in neuronal development, plasticity and disease.
Kaila et al. (2014)
16 more

1 mention without citation

Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.
Grimley et al. (2013)