3sq8

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase 1 H432R Mutant (SCAN1 Mutant)

Released:

Function and Biology Details

Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-153609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosyl-DNA phosphodiesterase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 54.15 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38319 (Residues: 79-539; Coverage: 85%)
Gene names: TDP1, YBR1520, YBR223C
Sequence domains: Tyrosyl-DNA phosphodiesterase
Structure domains: Endonuclease Chain A

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P1
Unit cell:
a: 63.871Å b: 81.492Å c: 96.787Å
α: 89.52° β: 84.9° γ: 67.82°
R-values:
R R work R free
0.189 0.187 0.226
Expression system: Escherichia coli BL21(DE3)