› 3spw

X-ray diffraction
2.6Å resolution

Structure of Osh4p/Kes1p in complex with phosphatidylinositol 4-phosphate

Released:
DOI: 10.2210/pdb3spw/pdb
PDB entry 3spw coloured by chain, front view.
PDB entry 3spw coloured by chain, side view.
PDB entry 3spw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers.
de Saint-Jean M, Delfosse V, Douguet D, Chicanne G, Payrastre B, Bourguet W, Antonny B, Drin G
J Cell Biol 195 965-78 (2011)
PMID: 22162133

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 49.7 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35844 (Residues: 1-434; Coverage: 100%)
Gene names: BSR3, KES1, LPI3C, OSH4, P2614, YPL145C
Sequence domains: Oxysterol-binding protein
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand T7M 2 x T7M
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 73.46Å b: 54.76Å c: 121.86Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.23 0.228 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

77 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
76 more

7 mentions without citation

Advances on the Transfer of Lipids by Lipid Transfer Proteins.
Wong et al. (2017)
6 more