› 3slt

X-ray diffraction
2.46Å resolution

Pre-cleavage Structure of the Autotransporter EspP - N1023S Mutant

Released:
DOI: 10.2210/pdb3slt/pdb
PDB entry 3slt coloured by chain, front view.
PDB entry 3slt coloured by chain, side view.
PDB entry 3slt coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.
Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK
J Mol Biol 415 128-42 (2012)
PMID: 22094314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autotransporter protein EspP translocator Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 34.43 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BSW5 (Residues: 999-1300; Coverage: 24%)
Gene names: ECO57PM78, L7020, espP
Sequence domains: Autotransporter beta-domain
Structure domains: Autotransporter beta-domain

Ligands and Environments

1 bound ligand:
Ligand C8E 10 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 31.162Å b: 122.372Å c: 122.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.266
Expression system: Escherichia coli

Quick links

Citations

7 review citations

From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis.
Leyton et al. (2012)
6 more

3 mentions without citation

Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence.
Ruiz-Perez et al. (2014)
2 more