› 3se2

X-ray diffraction
2.3Å resolution

Human poly(ADP-ribose) polymerase 14 (PARP14/ARTD8) - catalytic domain in complex with 6(5H)-phenanthridinone

Released:
DOI: 10.2210/pdb3se2/pdb
PDB entry 3se2 coloured by chain, front view.
PDB entry 3se2 coloured by chain, side view.
PDB entry 3se2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors.
Wahlberg E, Karlberg T, Kouznetsova E, Markova N, Macchiarulo A, Thorsell AG, Pol E, Frostell Å, Ekblad T, Öncü D, Kull B, Robertson GM, Pellicciari R, Schüler H, Weigelt J
Nat Biotechnol 30 283-8 (2012)
PMID: 22343925

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175098 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein mono-ADP-ribosyltransferase PARP14 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 193 amino acids
Theoretical weight: 22.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q460N5 (Residues: 1611-1801; Coverage: 11%)
Gene names: BAL2, KIAA1268, PARP14
Sequence domains: Poly(ADP-ribose) polymerase catalytic domain
Structure domains: Phosphoenolpyruvate Carboxykinase; domain 3

Ligands and Environments

5 bound ligands:
Ligand LDR 2 x LDR
Ligand GOL 4 x GOL
Ligand SCN 3 x SCN
Ligand 3AB 2 x 3AB
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P6522
Unit cell:
a: 82.372Å b: 82.372Å c: 434.478Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

92 review citations

New insights into the molecular and cellular functions of poly(ADP-ribose) and PARPs.
Gibson et al. (2012)
91 more

4 mentions without citation

Research Progress on PARP14 as a Drug Target.
Qin et al. (2019)
3 more