› 3s8h

X-ray diffraction
2.7Å resolution

Structure of dihydrodipicolinate synthase complexed with 3-Hydroxypropanoic acid(HPA)at 2.70 A resolution

Released:
DOI: 10.2210/pdb3s8h/pdb
PDB entry 3s8h coloured by chain, front view.
PDB entry 3s8h coloured by chain, side view.
PDB entry 3s8h coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Kumar M, Kaur N, Kumar S, Sinha M, Kaur P, Sharma S, Singh TP

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 31.42 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I4W3 (Residues: 1-292; Coverage: 100%)
Gene names: PA1010, dapA
Sequence domains: Dihydrodipicolinate synthetase family
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 3OH 2 x 3OH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 81.459Å b: 81.94Å c: 82.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.242
Expression system: Escherichia coli

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