› 3ru9

X-ray diffraction
2.21Å resolution

Specific recognition of N-acetylated substrates and domain flexibility in WbgU: a UDP-GalNAc 4-epimerase

Released:
DOI: 10.2210/pdb3ru9/pdb
PDB entry 3ru9 coloured by chain, front view.
PDB entry 3ru9 coloured by chain, side view.
PDB entry 3ru9 coloured by chain, top view.
Source organism: Plesiomonas shigelloides
Entry authors: Bhatt VS, Guan W, Wang PG

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181364 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 4-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 39.82 KDa
Source organism: Plesiomonas shigelloides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7BJX9 (Residues: 1-345; Coverage: 100%)
Gene name: wbgU
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand SO4 2 x SO4
Ligand UNL 4 x UNL
Ligand GLY 2 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 77.41Å b: 77.41Å c: 224.887Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.232
Expression system: Escherichia coli BL21(DE3)

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