› 3rsz

X-ray diffraction
3.01Å resolution

Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2

Released:
DOI: 10.2210/pdb3rsz/pdb
PDB entry 3rsz coloured by chain, front view.
PDB entry 3rsz coloured by chain, side view.
PDB entry 3rsz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Multiple glycogen-binding sites in eukaryotic glycogen synthase are required for high catalytic efficiency toward glycogen.
Baskaran S, Chikwana VM, Contreras CJ, Davis KD, Wilson WA, DePaoli-Roach AA, Roach PJ, Hurley TD
J Biol Chem 286 33999-4006 (2011)
PMID: 21835915

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 725 amino acids
Theoretical weight: 82.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27472 (Residues: 1-705; Coverage: 100%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase
Structure domains: Glycogen Phosphorylase B;
Glycogen [starch] synthase isoform 2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 96.557Å b: 166.731Å c: 121.136Å
α: 90° β: 103.25° γ: 90°
R-values:
R R work R free
0.208 0.207 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Glycogen and its metabolism: some new developments and old themes.
Roach et al. (2012)
2 more

1 mention without citation

Multiple glycogen-binding sites in eukaryotic glycogen synthase are required for high catalytic efficiency toward glycogen.
Baskaran et al. (2011)