› 3rqu

X-ray diffraction
3.09Å resolution

Crystal structure of a prokaryotic pentameric ligand-gated ion channel, ELIC

Released:
DOI: 10.2210/pdb3rqu/pdb
PDB entry 3rqu coloured by chain, front view.
PDB entry 3rqu coloured by chain, side view.
PDB entry 3rqu coloured by chain, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.
Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P
Nat Commun 3 714 (2012)
PMID: 22395605

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 322 amino acids
Theoretical weight: 36.88 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 22-343; Coverage: 100%)
Gene name: Dda3937_00520
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MES 7 x MES
Ligand GOL 21 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 105.289Å b: 266.97Å c: 111.074Å
α: 90° β: 107.53° γ: 90°
R-values:
R R work R free
0.211 0.209 0.24
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The nicotinic acetylcholine receptor: the founding father of the pentameric ligand-gated ion channel superfamily.
Changeux JP. (2012)
12 more

15 mentions without citation

A gating mechanism of pentameric ligand-gated ion channels.
Calimet et al. (2013)
14 more