› 3rp6

X-ray diffraction
2.2Å resolution

Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD

Released:
DOI: 10.2210/pdb3rp6/pdb
PDB entry 3rp6 coloured by chain, front view.
PDB entry 3rp6 coloured by chain, side view.
PDB entry 3rp6 coloured by chain, top view.
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Primary publication:
Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae.
Hicks KA, O'Leary SE, Begley TP, Ealick SE
Biochemistry 52 477-87 (2013)
PMID: 23259842

Function and Biology Details

Reaction catalysed: 
Urate + NADH + O(2) = 5-hydroxyisourate + NAD(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108050 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-dependent urate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 45.4 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Expression system: Escherichia coli
UniProt:
  • Canonical: A6T923 (Residues: 1-384; Coverage: 100%)
Gene names: KPN78578_16330, KPN_01663, hpxO
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 66.65Å b: 67.646Å c: 82.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.254
Expression system: Escherichia coli

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Citations

1 review citation

Flavin dependent monooxygenases.
Huijbers et al. (2014)
11 other articles

3 mentions without citation

Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid-assisted flavoprotein strategy for regioselective aromatic hydroxylation.
Montersino et al. (2013)
2 more