› 3rki

X-ray diffraction
3.2Å resolution

Structural basis for immunization with post-fusion RSV F to elicit high neutralizing antibody titers

Released:
DOI: 10.2210/pdb3rki/pdb
PDB entry 3rki coloured by chain, front view.
PDB entry 3rki coloured by chain, side view.
PDB entry 3rki coloured by chain, top view.
Source organism: Human orthopneumovirus
Primary publication:
Structural basis for immunization with postfusion respiratory syncytial virus fusion F glycoprotein (RSV F) to elicit high neutralizing antibody titers.
Swanson KA, Settembre EC, Shaw CA, Dey AK, Rappuoli R, Mandl CW, Dormitzer PR, Carfi A
Proc Natl Acad Sci U S A 108 9619-24 (2011)
PMID: 21586636

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fusion glycoprotein F0 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 528 amino acids
Theoretical weight: 58.49 KDa
Source organism: Human orthopneumovirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03420 (Residues: 1-524; Coverage: 89%)
Gene name: F
Sequence domains: Fusion glycoprotein F0
Structure domains: Helix Hairpins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 87.93Å b: 113.16Å c: 311.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

46 review citations

Progress in understanding and controlling respiratory syncytial virus: still crazy after all these years.
Collins et al. (2011)
45 more

14 mentions without citation

Protein Crystallography in Vaccine Research and Development.
Malito et al. (2015)
13 more