› 3r4i

X-ray diffraction
2.24Å resolution

Crystal structure of a Citrate lyase (Bxe_B2899) from BURKHOLDERIA XENOVORANS LB400 at 2.24 A resolution

Released:
DOI: 10.2210/pdb3r4i/pdb
PDB entry 3r4i coloured by chain, front view.
PDB entry 3r4i coloured by chain, side view.
PDB entry 3r4i coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Citrate = acetate + oxaloacetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171879 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HpcH/HpaI aldolase/citrate lyase domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 339 amino acids
Theoretical weight: 37.53 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13S43 (Residues: 1-338; Coverage: 100%)
Gene name: Bxe_B2899
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 13 x CA
Ligand CL 5 x CL
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3
Unit cell:
a: 148.468Å b: 148.468Å c: 79.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.174
Expression system: Escherichia coli

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