› 3qwu

X-ray diffraction
2.35Å resolution

Putative ATP-dependent DNA ligase from Aquifex aeolicus.

Released:
DOI: 10.2210/pdb3qwu/pdb
PDB entry 3qwu coloured by chain, front view.
PDB entry 3qwu coloured by chain, side view.
PDB entry 3qwu coloured by chain, top view.
Source organism: Aquifex aeolicus
Entry authors: Osipiuk J, Quartey P, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 43.61 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67191 (Residues: 1-367; Coverage: 100%)
Gene name: aq_1106
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADN 2 x ADN
Ligand CA 2 x CA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 91.366Å b: 99.165Å c: 103.278Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.255
Expression system: Escherichia coli BL21(DE3)

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