› 3qo7

X-ray diffraction
2.55Å resolution

Crystal structure of the seryl-tRNA synthetase from Candida albicans

Released:
DOI: 10.2210/pdb3qo7/pdb
PDB entry 3qo7 coloured by chain, front view.
PDB entry 3qo7 coloured by chain, side view.
PDB entry 3qo7 coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
Unveiling the structural basis for translational ambiguity tolerance in a human fungal pathogen.
Rocha R, Pereira PJ, Santos MA, Macedo-Ribeiro S
Proc Natl Acad Sci U S A 108 14091-6 (2011)
PMID: 21825144

Function and Biology Details

Reaction catalysed: 
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 55.68 KDa
Source organism: Candida albicans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HGT6 (Residues: 1-462; Coverage: 100%)
Gene names: CAALFM_C302780WA, CaO19.7901, SES1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6122
Unit cell:
a: 91.152Å b: 91.152Å c: 275.839Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

The Candida pathogenic species complex.
Turner et al. (2014)
6 more

1 mention without citation

Unveiling the structural basis for translational ambiguity tolerance in a human fungal pathogen.
Rocha et al. (2011)