› 3qim

X-ray diffraction
2.1Å resolution

Histidine 416 of the periplamsic binding protein NikA is essential for nickel uptake in Escherichia coli

Released:
DOI: 10.2210/pdb3qim/pdb
PDB entry 3qim coloured by chain, front view.
PDB entry 3qim coloured by chain, side view.
PDB entry 3qim coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Histidine 416 of the periplasmic binding protein NikA is essential for nickel uptake in Escherichia coli.
Cavazza C, Martin L, Laffly E, Lebrette H, Cherrier MV, Zeppieri L, Richaud P, Carrière M, Fontecilla-Camps JC
FEBS Lett 585 711-5 (2011)
PMID: 21281641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 56.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P33590 (Residues: 23-524; Coverage: 100%)
Gene names: JW3441, b3476, nikA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 8 x GOL
Ligand ACT 13 x ACT
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 86.731Å b: 94.005Å c: 124.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.237
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein conformational switches: from nature to design.
Ha et al. (2012)
3 more