› 3qbz

X-ray diffraction
2.69Å resolution

Crystal structure of the Rad53-recognition domain of Saccharomyces cerevisiae Dbf4

Released:
DOI: 10.2210/pdb3qbz/pdb
PDB entry 3qbz coloured by chain, front view.
PDB entry 3qbz coloured by chain, side view.
PDB entry 3qbz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Saccharomyces cerevisiae Dbf4 has unique fold necessary for interaction with Rad53 kinase.
Matthews LA, Jones DR, Prasad AA, Duncker BP, Guarné A
J Biol Chem 287 2378-87 (2012)
PMID: 22130670

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DDK kinase regulatory subunit DBF4 Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 18.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32325 (Residues: 66-221; Coverage: 22%)
Gene names: D4205, DBF4, DNA52, YD9609.07C, YDR052C

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6422
Unit cell:
a: 83.74Å b: 83.74Å c: 103.669Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

FHA domains: Phosphopeptide binding and beyond.
Almawi et al. (2017)
2 more

8 mentions without citation

DNA replication checkpoint signaling depends on a Rad53-Dbf4 N-terminal interaction in Saccharomyces cerevisiae.
Chen et al. (2013)
7 more