› 3q7e

X-ray diffraction
2.2Å resolution

Crystal Structure of rat Protein Arginine Methyltransferase 1 (PRMT1)M48L mutant

Released:
DOI: 10.2210/pdb3q7e/pdb
PDB entry 3q7e coloured by chain, front view.
PDB entry 3q7e coloured by chain, side view.
PDB entry 3q7e coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Investigation of the molecular origins of protein-arginine methyltransferase I (PRMT1) product specificity reveals a role for two conserved methionine residues.
Gui S, Wooderchak WL, Daly MP, Porter PJ, Johnson SJ, Hevel JM
J Biol Chem 286 29118-29126 (2011)
PMID: 21697082

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein arginine N-methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63009 (Residues: 14-353; Coverage: 96%)
Gene names: Hrmt1l2, Prmt1
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SAH 1 x SAH
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4122
Unit cell:
a: 87.307Å b: 87.307Å c: 143.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.245
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Biochemistry and regulation of the protein arginine methyltransferases (PRMTs).
Morales et al. (2016)
4 more

7 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
6 more