› 3q4z

X-ray diffraction
1.89Å resolution

Structure of unphosphorylated PAK1 kinase domain

Released:
DOI: 10.2210/pdb3q4z/pdb
PDB entry 3q4z coloured by chain, front view.
PDB entry 3q4z coloured by chain, side view.
PDB entry 3q4z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the autoactivation mechanism of p21-activated protein kinase.
Wang J, Wu JW, Wang ZX
Structure 19 1752-61 (2011)
PMID: 22153498

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171570 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PAK 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13153 (Residues: 248-545; Coverage: 55%)
Gene name: PAK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.988Å b: 79.996Å c: 65.664Å
α: 90° β: 107.51° γ: 90°
R-values:
R R work R free
0.186 0.184 0.23
Expression system: Escherichia coli

Quick links

Citations

13 review citations

How Do Protein Kinases Take a Selfie (Autophosphorylate)?
Beenstock et al. (2016)
12 more

5 mentions without citation

Identifying three-dimensional structures of autophosphorylation complexes in crystals of protein kinases.
Xu et al. (2015)
4 more