› 3pzw

X-ray diffraction
1.4Å resolution

Soybean lipoxygenase-1 - re-refinement

Released:
DOI: 10.2210/pdb3pzw/pdb
PDB entry 3pzw coloured by chain, front view.
PDB entry 3pzw coloured by chain, side view.
PDB entry 3pzw coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Determination of protein structures--a series of fortunate events.
Chruszcz M, Wlodawer A, Minor W
Biophys J 95 1-9 (2008)
PMID: 18441029

Function and Biology Details

Reaction catalysed: 
Alpha-linolenate + O(2) = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 13S-lipoxygenase-1 Chain: A
Molecule details ›
Chain: A
Length: 839 amino acids
Theoretical weight: 94.52 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: P08170 (Residues: 1-839; Coverage: 100%)
Gene names: LOX1, LOX1.1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FE2 1 x FE2
Ligand EDO 4 x EDO
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 94.9Å b: 94Å c: 49.9Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.15 0.149 0.169
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The future of crystallography in drug discovery.
Zheng et al. (2014)
9 more

29 mentions without citation

Understanding Biological Hydrogen Transfer Through the Lens of Temperature Dependent Kinetic Isotope Effects.
Klinman et al. (2018)
28 more