› 3pzk

X-ray diffraction
2.23Å resolution

Crystal Structure of the Mycobacterium tuberculosis crotonase in apo form

Released:
DOI: 10.2210/pdb3pzk/pdb
PDB entry 3pzk coloured by chain, front view.
PDB entry 3pzk coloured by chain, side view.
PDB entry 3pzk coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Bruning JB, Delgado E, Ghosh S, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-161871 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable enoyl-CoA hydratase EchA8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 257 amino acids
Theoretical weight: 27.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNN9 (Residues: 1-257; Coverage: 100%)
Gene names: MTV017.23c, Rv1070c, echA8
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 133.796Å b: 133.734Å c: 103.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.238
Expression system: Escherichia coli BL21(DE3)

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