› 3pwt

X-ray diffraction
1.9Å resolution

Crystal structure of mutant E.coli topoisomerase IA

Released:
DOI: 10.2210/pdb3pwt/pdb
PDB entry 3pwt coloured by chain, front view.
PDB entry 3pwt coloured by chain, side view.
PDB entry 3pwt coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Zhang Z, Bokun C, Tse-Dinh Y-C

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 67.28 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06612 (Residues: 1-596; Coverage: 69%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 63.493Å b: 79.263Å c: 141.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.249
Expression system: Escherichia coli BL21

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