› 3paf

X-ray diffraction
1.7Å resolution

M. jannaschii L7Ae mutant

Released:
DOI: 10.2210/pdb3paf/pdb
PDB entry 3paf coloured by chain, front view.
PDB entry 3paf coloured by chain, side view.
PDB entry 3paf coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry authors: Biswas S, Maxwell ES

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large ribosomal subunit protein eL8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 12.73 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54066 (Residues: 1-117; Coverage: 100%)
Gene names: MJ1203, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 43.443Å b: 55.634Å c: 103.039Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.208
Expression system: Escherichia coli BL21(DE3)

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