› 3p35

X-ray diffraction
2.09Å resolution

Polo-like kinase I Polo-box domain in complex with MQSpSPL phosphopeptide

Released:
DOI: 10.2210/pdb3p35/pdb
PDB entry 3p35 coloured by chain, front view.
PDB entry 3p35 coloured by chain, side view.
PDB entry 3p35 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
From crystal packing to molecular recognition: prediction and discovery of a binding site on the surface of polo-like kinase 1.
Śledź P, Stubbs CJ, Lang S, Yang YQ, McKenzie GJ, Venkitaraman AR, Hyvönen M, Abell C
Angew Chem Int Ed Engl 50 4003-6 (2011)
PMID: 21472932

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-156761 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 232 amino acids
Theoretical weight: 26.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 371-594; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
phosphopeptide Chains: D, E
Molecule details ›
Chains: D, E
Length: 8 amino acids
Theoretical weight: 766 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 58.893Å b: 95.828Å c: 65.964Å
α: 90° β: 116.34° γ: 90°
R-values:
R R work R free
0.209 0.206 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Small molecules, big targets: drug discovery faces the protein-protein interaction challenge.
Scott et al. (2016)
6 more

5 mentions without citation

From crystal packing to molecular recognition: prediction and discovery of a binding site on the surface of polo-like kinase 1.
Śledź et al. (2011)
4 more