› 3p28

X-ray diffraction
1.8Å resolution

Structure of a Circular Permutant of Green Fluorescent Protein

Released:
DOI: 10.2210/pdb3p28/pdb
PDB entry 3p28 coloured by chain, front view.
PDB entry 3p28 coloured by chain, side view.
PDB entry 3p28 coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Melief EH, Kim H, Kim TS, Wachter RM, Tonge PJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.24 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 50-229, 3-49; Coverage: 95%)
Gene name: GFP
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYS 1 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 41.456Å b: 71.028Å c: 76.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.237
Expression system: Escherichia coli BL21(DE3)

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