› 3p1g

X-ray diffraction
1.5Å resolution

Crystal Structure of the Xenotropic Murine Leukemia Virus-Related Virus (XMRV) RNase H Domain

Released:
DOI: 10.2210/pdb3p1g/pdb
PDB entry 3p1g coloured by chain, front view.
PDB entry 3p1g coloured by chain, side view.
PDB entry 3p1g coloured by chain, top view.
Source organism: Xenotropic MuLV-related virus
Primary publication:
Structural and inhibition studies of the RNase H function of xenotropic murine leukemia virus-related virus reverse transcriptase.
Kirby KA, Marchand B, Ong YT, Ndongwe TP, Hachiya A, Michailidis E, Leslie MD, Sietsema DV, Fetterly TL, Dorst CA, Singh K, Wang Z, Parniak MA, Sarafianos SG
Antimicrob Agents Chemother 56 2048-61 (2012)
PMID: 22252812

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase/ribonuclease H p80 Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 17.97 KDa
Source organism: Xenotropic MuLV-related virus
Expression system: Escherichia coli
UniProt:
  • Canonical: A1Z651 (Residues: 1155-1328; Coverage: 9%)
Gene name: gag-pol
Sequence domains: RNase H
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P41
Unit cell:
a: 37.53Å b: 37.53Å c: 100.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.182
Expression system: Escherichia coli

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Citations

2 review citations

"Old Dogs with New Tricks": exploiting alternative mechanisms of action and new drug design strategies for clinically validated HIV targets.
Kang et al. (2014)
1 more

6 mentions without citation

Structural and inhibition studies of the RNase H function of xenotropic murine leukemia virus-related virus reverse transcriptase.
Kirby et al. (2012)
5 more