› 3p0b

X-ray diffraction
1.35Å resolution

Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed

Released:
DOI: 10.2210/pdb3p0b/pdb
PDB entry 3p0b coloured by chain, front view.
PDB entry 3p0b coloured by chain, side view.
PDB entry 3p0b coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Pijning T, Dijkstra BW

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-alpha-glucan branching enzyme TTHA1902 Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.42 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SH28 (Residues: 1-520; Coverage: 100%)
Gene names: TT1467, TTHA1902
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P42212
Unit cell:
a: 119.532Å b: 119.532Å c: 74.097Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.189
Expression system: Escherichia coli

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