› 3oqm

X-ray diffraction
2.96Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116662 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Catabolite control protein A Chains: A, C
Molecule details ›
Chains: A, C
Length: 339 amino acids
Theoretical weight: 37.72 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P25144 (Residues: 3-334; Coverage: 99%)
Gene names: BSU29740, alsA, amyR, ccpA, graR
Sequence domains:
Structure domains:
Phosphocarrier protein HPr Chains: D, S
Molecule details ›
Chains: D, S
Length: 87 amino acids
Theoretical weight: 9.15 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08877 (Residues: 2-88; Coverage: 99%)
Gene names: BSU13900, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like
5'-D(*TP*TP*GP*TP*AP*AP*GP*CP*GP*TP*TP*AP*TP*CP*AP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
5'-D(*TP*TP*GP*AP*TP*AP*AP*CP*GP*CP*TP*TP*AP*CP*AP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.88 KDa

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 74.24Å b: 103.54Å c: 176.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.271
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Regulation of carbohydrate degradation pathways in Pseudomonas involves a versatile set of transcriptional regulators.
Udaondo et al. (2018)
29 other articles

5 mentions without citation

Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators.
Schumacher et al. (2011)
4 more