› 3ool

X-ray diffraction
2.3Å resolution

I-SceI complexed with C/G+4 DNA substrate

Released:
DOI: 10.2210/pdb3ool/pdb
PDB entry 3ool coloured by chain, front view.
PDB entry 3ool coloured by chain, side view.
PDB entry 3ool coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.
Joshi R, Ho KK, Tenney K, Chen JH, Golden BL, Gimble FS
J Mol Biol 405 185-200 (2011)
PMID: 21029741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116658 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Intron-encoded endonuclease I-SceI Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 27.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03882 (Residues: 1-235; Coverage: 100%)
Gene names: OMEGA, Q0160, SCEI, SECY
Sequence domains: LAGLIDADG DNA endonuclease family
Structure domains: Homing endonucleases
5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.64 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 129.184Å b: 49.938Å c: 63.36Å
α: 90° β: 107.54° γ: 90°
R-values:
R R work R free
0.327 0.246 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

CRISPR/Cas9 advances engineering of microbial cell factories.
Jakočiūnas et al. (2016)
3 more

1 mention without citation

Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.
Joshi et al. (2011)