› 3odf

X-ray diffraction
1.1Å resolution

Comparison of the character and the speed of X-ray-induced structural changes of porcine pancreatic elastase at two temperatures, 100 and 15K. The data set was collected from region A of the crystal. Second step of radiation damage

Released:
DOI: 10.2210/pdb3odf/pdb
PDB entry 3odf coloured by chain, front view.
PDB entry 3odf coloured by chain, side view.
PDB entry 3odf coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray-induced deterioration of disulfide bridges at atomic resolution.
Petrova T, Ginell S, Mitschler A, Kim Y, Lunin VY, Joachimiak G, Cousido-Siah A, Hazemann I, Podjarny A, Lazarski K, Joachimiak A
Acta Crystallogr D Biol Crystallogr 66 1075-91 (2010)
PMID: 20944241

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymotrypsin-like elastase family member 1 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.93 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00772 (Residues: 27-266; Coverage: 96%)
Gene names: CELA1, ELA1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 50.034Å b: 57.742Å c: 74.449Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.122 0.147

Quick links

Citations

7 citation in other articles

Crystal structure of lactose permease in complex with an affinity inactivator yields unique insight into sugar recognition.
Chaptal et al. (2011)
6 more