› 3od2

X-ray diffraction
2.6Å resolution

E. coli NikR soaked with excess nickel ions

Released:
DOI: 10.2210/pdb3od2/pdb
PDB entry 3od2 coloured by chain, front view.
PDB entry 3od2 coloured by chain, side view.
PDB entry 3od2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli.
Phillips CM, Schreiter ER, Stultz CM, Drennan CL
Biochemistry 49 7830-8 (2010)
PMID: 20704276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Z6 (Residues: 1-133; Coverage: 100%)
Gene names: JW3446, b3481, nikR, yhhG
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 11 x NI
Ligand 3CM 1 x 3CM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P3121
Unit cell:
a: 50.735Å b: 50.735Å c: 183.114Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.286
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metal site occupancy and allosteric switching in bacterial metal sensor proteins.
Guerra et al. (2012)
1 more

2 mentions without citation

Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli.
Phillips et al. (2010)
1 more