› 3och

X-ray diffraction
1.79Å resolution

Chemically Self-assembled Antibody Nanorings (CSANs): Design and Characterization of an Anti-CD3 IgM Biomimetic

Released:
DOI: 10.2210/pdb3och/pdb
PDB entry 3och coloured by chain, front view.
PDB entry 3och coloured by chain, side view.
PDB entry 3och coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Chemically self-assembled antibody nanorings (CSANs): design and characterization of an anti-CD3 IgM biomimetic.
Li Q, So CR, Fegan A, Cody V, Sarikaya M, Vallera DA, Wagner CR
J Am Chem Soc 132 17247-57 (2010)
PMID: 21077608

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 18.02 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABQ4 (Residues: 1-159; Coverage: 100%)
Gene names: JW0047, b0048, folA, tmrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

3 bound ligands:
Ligand CL 3 x CL
Ligand 2MX 1 x 2MX
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P61
Unit cell:
a: 91.714Å b: 91.714Å c: 73.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.242
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Cancer nanotheranostics: improving imaging and therapy by targeted delivery across biological barriers.
Kievit et al. (2011)
4 more

5 mentions without citation

Chemically self-assembled antibody nanorings (CSANs): design and characterization of an anti-CD3 IgM biomimetic.
Li et al. (2010)
4 more