› 3o1c

X-ray diffraction
1.08Å resolution

High resolution crystal structure of histidine triad nucleotide-binding protein 1 (Hint1) C38A mutant from rabbit complexed with Adenosine

Released:
DOI: 10.2210/pdb3o1c/pdb
PDB entry 3o1c coloured by chain, front view.
PDB entry 3o1c coloured by chain, side view.
PDB entry 3o1c coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Histidine triad nucleotide-binding protein 1 (HINT-1) phosphoramidase transforms nucleoside 5'-O-phosphorothioates to nucleoside 5'-O-phosphates.
Ozga M, Dolot R, Janicka M, Kaczmarek R, Krakowiak A
J Biol Chem 285 40809-18 (2010)
PMID: 20940308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 13.68 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P80912 (Residues: 1-126; Coverage: 100%)
Gene names: HINT, HINT1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

2 bound ligands:
Ligand ADN 1 x ADN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 39.965Å b: 39.965Å c: 141.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.163
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Hydrogen sulfide in pharmacology and medicine--An update.
Bełtowski J. (2015)
5 more

4 mentions without citation

Strategies for Sustainable Production of Hydrogen Peroxide via Oxygen Reduction Reaction: From Catalyst Design to Device Setup.
Tian et al. (2023)
3 more