› 3ny2

X-ray diffraction
2.61Å resolution

Structure of the ubr-box of UBR2 ubiquitin ligase

Released:
DOI: 10.2210/pdb3ny2/pdb
PDB entry 3ny2 coloured by chain, front view.
PDB entry 3ny2 coloured by chain, side view.
PDB entry 3ny2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of substrate recognition and specificity in the N-end rule pathway.
Matta-Camacho E, Kozlov G, Li FF, Gehring K
Nat Struct Mol Biol 17 1182-7 (2010)
PMID: 20835242

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase UBR2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 75 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IWV8 (Residues: 98-167; Coverage: 4%)
Gene names: C6orf133, KIAA0349, UBR2
Sequence domains: Putative zinc finger in N-recognin (UBR box)

Ligands and Environments

1 bound ligand:
Ligand ZN 24 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 29.39Å b: 61.456Å c: 72.806Å
α: 65.05° β: 89.98° γ: 90.01°
R-values:
R R work R free
0.24 0.23 0.288
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

AAA+ proteases: ATP-fueled machines of protein destruction.
Sauer et al. (2011)
13 more

1 mention without citation

The UBR-box and its relationship to binuclear RING-like treble clef zinc fingers.
Kaur et al. (2015)