› 3nss

X-ray diffraction
1.9Å resolution

The 2009 pandemic H1N1 neuraminidase N1 lacks the 150-cavity in its active sites

Released:
DOI: 10.2210/pdb3nss/pdb
PDB entry 3nss coloured by chain, front view.
PDB entry 3nss coloured by chain, side view.
PDB entry 3nss coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
The 2009 pandemic H1N1 neuraminidase N1 lacks the 150-cavity in its active site.
Li Q, Qi J, Zhang W, Vavricka CJ, Shi Y, Wei J, Feng E, Shen J, Chen J, Liu D, He J, Yan J, Liu H, Jiang H, Teng M, Li X, Gao GF
Nat Struct Mol Biol 17 1266-8 (2010)
PMID: 20852645

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.73 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: C3W5S3 (Residues: 82-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 5 x CA
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 118.537Å b: 137.857Å c: 118.512Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.169 0.196
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Advances in the development of influenza virus vaccines.
Krammer et al. (2015)
12 more

48 mentions without citation

Contribution of antibody production against neuraminidase to the protection afforded by influenza vaccines.
Marcelin et al. (2012)
47 more