› 3nou

X-ray diffraction
3Å resolution

Light-induced intermediate structure L3 of P. aeruginosa bacteriophytochrome

Released:
DOI: 10.2210/pdb3nou/pdb
PDB entry 3nou coloured by chain, front view.
PDB entry 3nou coloured by chain, side view.
PDB entry 3nou coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome.
Yang X, Ren Z, Kuk J, Moffat K
Nature 479 428-32 (2011)
PMID: 22002602

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: C
Molecule details ›
Chain: C
Length: 505 amino acids
Theoretical weight: 56.82 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HWR3 (Residues: 1-499; Coverage: 69%)
Gene names: PA4117, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BLA 1 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 154.38Å b: 162.979Å c: 436.098Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Phytochromes: an atomic perspective on photoactivation and signaling.
Burgie et al. (2014)
7 more