› 3nnc

X-ray diffraction
2.2Å resolution

Crystal Structure of CUGBP1 RRM1/2-RNA Complex

Released:
DOI: 10.2210/pdb3nnc/pdb
PDB entry 3nnc coloured by chain, front view.
PDB entry 3nnc coloured by chain, side view.
PDB entry 3nnc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into RNA recognition by the alternate-splicing regulator CUG-binding protein 1.
Teplova M, Song J, Gaw HY, Teplov A, Patel DJ
Structure 18 1364-77 (2010)
PMID: 20947024

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-187795 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CUGBP Elav-like family member 1 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92879 (Residues: 14-187; Coverage: 36%)
Gene names: BRUNOL2, CELF1, CUGBP, CUGBP1, NAB50
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*UP*GP*UP*GP*UP*GP*UP*UP*GP*UP*GP*UP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.17 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 47.713Å b: 70.039Å c: 132.548Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

The importance of CELF control: molecular and biological roles of the CUG-BP, Elav-like family of RNA-binding proteins.
Dasgupta et al. (2012)
12 more

3 mentions without citation

Comprehensive Identification of RNA-Binding Domains in Human Cells.
Castello et al. (2016)
2 more