› 3niq

X-ray diffraction
2.07Å resolution

Crystal structure of Pseudomonas aeruginosa guanidinopropionase

Released:
DOI: 10.2210/pdb3niq/pdb
PDB entry 3niq coloured by chain, front view.
PDB entry 3niq coloured by chain, side view.
PDB entry 3niq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily.
Lee SJ, Kim DJ, Kim HS, Lee BI, Yoon HJ, Yoon JY, Kim KH, Jang JY, Im HN, An DR, Song JS, Kim HJ, Suh SW
J Struct Biol 175 329-38 (2011)
PMID: 21600989

Function and Biology Details

Reaction catalysed: 
3-guanidinopropanoate + H(2)O = beta-alanine + urea
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanidinopropionase Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.3 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6K2 (Residues: 1-318; Coverage: 100%)
Gene names: PA0288, gpuA
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P213
Unit cell:
a: 123.961Å b: 123.961Å c: 123.961Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.228
Expression system: Escherichia coli

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Citations

10 citation in other articles

An Amidinohydrolase Provides the Missing Link in the Biosynthesis of Amino Marginolactone Antibiotics.
Hong et al. (2016)
9 more