› 3nh1

X-ray diffraction
2.11Å resolution

Crystal structure of RNase T in complex with a preferred ssDNA (TAGG) with two Mg in the active site

Released:
DOI: 10.2210/pdb3nh1/pdb
PDB entry 3nh1 coloured by chain, front view.
PDB entry 3nh1 coloured by chain, side view.
PDB entry 3nh1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.
Hsiao YY, Yang CC, Lin CL, Lin JL, Duh Y, Yuan HS
Nat Chem Biol 7 236-43 (2011)
PMID: 21317904

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116620 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease T Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 25.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P30014 (Residues: 1-215; Coverage: 100%)
Gene names: JW1644, b1652, rnt
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H
5'-D(*TP*TP*AP*TP*AP*GP*G)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 7 nucleotides
Theoretical weight: 2.15 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P32
Unit cell:
a: 46.482Å b: 46.482Å c: 315.877Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.171 0.229
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Bacterial ribonucleases and their roles in RNA metabolism.
Bechhofer et al. (2019)
2 more

6 mentions without citation

Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase-RNA complexes.
Jones et al. (2022)
5 more