› 3nfh

X-ray diffraction
2.17Å resolution

Crystal structure of tandem winged helix domain of RNA polymerase I subunit A49 (P4)

Released:
DOI: 10.2210/pdb3nfh/pdb
PDB entry 3nfh coloured by chain, front view.
PDB entry 3nfh coloured by chain, side view.
PDB entry 3nfh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
RNA polymerase I contains a TFIIF-related DNA-binding subcomplex.
Geiger SR, Lorenzen K, Schreieck A, Hanecker P, Kostrewa D, Heck AJ, Cramer P
Mol Cell 39 583-94 (2010)
PMID: 20797630

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase I subunit RPA49 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01080 (Residues: 154-399; Coverage: 59%)
Gene names: N0880, RPA49, RRN13, YNL248C
Sequence domains: A49-like RNA polymerase I associated factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43
Unit cell:
a: 96.71Å b: 96.71Å c: 54.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.224
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Ribosome biogenesis in the yeast Saccharomyces cerevisiae.
Woolford et al. (2013)
28 more