› 3nep

X-ray diffraction
1.55Å resolution

1.55A resolution structure of malate dehydrogenase from Salinibacter ruber

Released:
DOI: 10.2210/pdb3nep/pdb
PDB entry 3nep coloured by chain, front view.
PDB entry 3nep coloured by chain, side view.
PDB entry 3nep coloured by chain, top view.
Source organism: Salinibacter ruber DSM 13855
Primary publication:
Gradual adaptive changes of a protein facing high salt concentrations.
Coquelle N, Talon R, Juers DH, Girard E, Kahn R, Madern D
J Mol Biol 404 493-505 (2010)
PMID: 20888835

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chain: X
Molecule details ›
Chain: X
Length: 314 amino acids
Theoretical weight: 33.29 KDa
Source organism: Salinibacter ruber DSM 13855
UniProt:
  • Canonical: Q2S289 (Residues: 1-314; Coverage: 100%)
Gene names: SRU_1571, mdh
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A, ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 76.11Å b: 87.72Å c: 100.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.192

Quick links

Citations

3 review citations

Halophiles and their enzymes: negativity put to good use.
DasSarma et al. (2015)
2 more

1 mention without citation

Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein.
Mahatabuddin et al. (2018)