› 3n0a

X-ray diffraction
2.2Å resolution

Crystal structure of auxilin (40-400)

Released:
DOI: 10.2210/pdb3n0a/pdb
PDB entry 3n0a coloured by chain, front view.
PDB entry 3n0a coloured by chain, side view.
PDB entry 3n0a coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of the PTEN-like region of auxilin, a detector of clathrin-coated vesicle budding.
Guan R, Dai H, Harrison SC, Kirchhausen T, Kirchhausen T
Structure 18 1191-8 (2010)
PMID: 20826345

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Auxilin Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.44 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q27974 (Residues: 40-400; Coverage: 40%)
Gene name: DNAJC6
Sequence domains: C2 domain of PTEN tumour-suppressor protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3221
Unit cell:
a: 137.925Å b: 137.925Å c: 51.327Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.2
Expression system: Not provided

Quick links

Citations

11 review citations

Molecular structure, function, and dynamics of clathrin-mediated membrane traffic.
Kirchhausen et al. (2014)
10 more

4 mentions without citation

The Role of Co-chaperones in Synaptic Proteostasis and Neurodegenerative Disease.
Gorenberg et al. (2017)
3 more