› 3mvu

X-ray diffraction
1.8Å resolution

Crystal structure of a TenA family transcription regulator (TM1040_3656) from SILICIBACTER SP. TM1040 at 1.80 A resolution

Released:
DOI: 10.2210/pdb3mvu/pdb
PDB entry 3mvu coloured by chain, front view.
PDB entry 3mvu coloured by chain, side view.
PDB entry 3mvu coloured by chain, top view.
Source organism: Ruegeria sp. TM1040
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminopyrimidine aminohydrolase Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.2 KDa
Source organism: Ruegeria sp. TM1040
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GL43 (Residues: 1-225; Coverage: 100%)
Gene name: TM1040_3656
Sequence domains: TENA/THI-4/PQQC family
Structure domains: Heme oxygenase-like

Ligands and Environments

3 bound ligands:
Ligand IMD 1 x IMD
Ligand EDO 12 x EDO
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P42212
Unit cell:
a: 65.315Å b: 65.315Å c: 107.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 0.179
Expression system: Escherichia coli

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