› 3mix

X-ray diffraction
2.3Å resolution

Crystal structure of the cytosolic domain of B. subtilis FlhA

Released:
DOI: 10.2210/pdb3mix/pdb
PDB entry 3mix coloured by chain, front view.
PDB entry 3mix coloured by chain, side view.
PDB entry 3mix coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system.
Bange G, Kümmerer N, Engel C, Bozkurt G, Wild K, Sinning I
Proc Natl Acad Sci U S A 107 11295-300 (2010)
PMID: 20534509

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar biosynthesis protein FlhA Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.8 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35620 (Residues: 303-677; Coverage: 55%)
Gene names: BSU16390, flhA
Sequence domains: FHIPEP family
Structure domains: FHIPEP family, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, ESRF BEAMLINE ID14-2
Spacegroup: P65
Unit cell:
a: 84.177Å b: 84.177Å c: 118.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
17 more

3 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
2 more