› 3mac

X-ray diffraction
2.5Å resolution

crystal structure of GP41-derived protein complexed with fab 8062

Released:
DOI: 10.2210/pdb3mac/pdb
PDB entry 3mac coloured by chain, front view.
PDB entry 3mac coloured by chain, side view.
PDB entry 3mac coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of HIV-1 neutralization by affinity matured Fabs directed against the internal trimeric coiled-coil of gp41.
Gustchina E, Li M, Louis JM, Anderson DE, Lloyd J, Frisch C, Bewley CA, Gustchina A, Wlodawer A, Clore GM
PLoS Pathog 6 e1001182 (2010)
PMID: 21085615

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207750 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transmembrane glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.48 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWW0 (Residues: 1-217; Coverage: 96%)
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Fab8062 Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 26.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Fab8062 Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.64 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 83.668Å b: 41.741Å c: 98.602Å
α: 90° β: 94.85° γ: 90°
R-values:
R R work R free
0.202 0.2 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Rational design of vaccines to elicit broadly neutralizing antibodies to HIV-1.
Kwong et al. (2011)
6 more

8 mentions without citation

HIV-1 envelope glycoprotein structure.
Merk et al. (2013)
7 more